Comparison of parasite count in intestine (IN), tongue (TO), heart (HE), quadriceps femoris (QF), lung (LU), intercostal muscles (IC), deltoid (DE), brain (BR) and diaphragm (DI), in naturally (A, B) and experimentally (C, D) infected lambs, in fresh (A, C) or frozen (B, D) samples. (Parasite count is expressed as the arithmetic mean of T. gondii parasites per gram of tissues. Each dot represents one lamb, red dot = E1, blue dot = E2, green dot = E3; horizontal line represents the mean of the parasite count per gram of tissue for the three lambs). The number of T. gondii parasites was calculated by interpolating the average Ct values on the standard curve equivalent to 1 × 10⁰ to 1 × 10³ tachyzoites with tenfold serial dilutions. Estimated parasite numbers in tissue samples (parasite count) were expressed as parasite number/g lamb tissue.