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Figure 3

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CYP450MO overexpression in Acanthamoeba (ATCC_30010). (A) Schematic of the pGAPDH-EGFP-CYP450MO vector. (B) Genomic DNA of Acanthamoeba transfected in the pGAPDH-EGFP-CYP450MO vector detected by PCR. (C) Acanthamoeba transfected with pGAPDH-EGFP and pGAPDH-EGFP-CYP450MO vector (green) incubated for 7 days and examined using a fluorescence microscope.

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