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Antibody response in vaccinated cattle. Bovine serum antibody levels to recombinant antigens were determined by ELISA in cattle vaccinated with rGluCl, rGlyR and adjuvant/saline control. Antibody levels in immunized cattle were expressed as OD450 nm values and compared between vaccinated and control cattle using an ANOVA test (*p < 0.05). The time of vaccination shots (arrows) and tick infestations (dashed line) are indicated.

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