Immunological response in mice vaccinated with the recombinant antigens. A. Immunization and bleeding scheme. B. Immunoreactivity of rGluCl verified by Western blotting. Lane M: protein marker; Lane 1: pooled preimmune sera from 4 mice; Lanes 2–5: individual sera of bleeding 3 from 4 mice; Lanes 6–9: individual sera of bleeding 4 from 4 mice. Immunoreactive bands of ~35 kDa are indicated by rectangles. C. Immunoreactivity of rGlyR verified by Western blotting. Lane M: protein marker; Lane 1: pooled preimmune sera from 3 mice; Lane 2: pooled sera of bleeding 2 from 3 mice; Lanes 3–5: individual sera of bleeding 3 from 3 mice; Lanes 6–8: individual sera of bleeding 4 from 3 mice. Immunoreactive bands of ~51 kDa are indicated by rectangles.