Recombinant proteins expression. A. SDS-PAGE analysis of protein fractions from E. coli cultures for rGluCl expression. M = Molecular weight marker. 1 = Uninduced and 2 = Induced culture with IPTG. B. Western blot analysis of rGluCl. M = Molecular weight marker. 1 = Uninduced and 2 = Induced culture with IPTG. C. SDS-PAGE analysis of protein fractions from E. coli cultures for rGlyR expression. M = Molecular weight marker. 1 = Uninduced and 2 = Induced culture with IPTG. D. Western blot analysis of rGlyR. M = Molecular weight marker. 1 = Uninduced and 2 = Induced culture with IPTG. In all cases, the molecular weight marker used was the Precision Plus Protein All Blue Prestained Protein Standard from Bio-Rad. Arrows indicate the size of the recombinant proteins.