Table 4
DNA measurements for the supplementary DNA extraction methods.
| Method Name | Sample ID | Average contamination Ratio (260/230) | Average purity ratio (260/280) | Average yield (ng/mL) |
|---|---|---|---|---|
| Magnetic Beads Kit | PEK DT | 0.76 | 1.08 | 33.67 |
| PEK F | 0.72 | 1.12 | 115 | |
| Farm IV DT | 0.66 | 1.06 | 40.65 | |
| Farm IVF | 0.68 | 1.09 | 24.82 | |
| Farm VIDT | 0.71 | 1.08 | 27.42 | |
| Farm VIF | 0.61 | 1.05 | 18.32 | |
| Chelex® Method 1 | PEK DT C10 | NA | NA | NA |
| PEK DT C20 | NA | NA | NA | |
| Farm IVDT C10 | 0.65 | 1.09 | 181.87 | |
| Farm IVDT C20 | 0.65 | 1.09 | 207.32 | |
| Farm VIDT C10 | 0.67 | 1.14 | 99.47 | |
| Farm VIDT C20 | 0.67 | 1.13 | 106.27 | |
| Chelex® Method 2 | PEK F | 0.59 | 0.95 | 34.45 |
| Farm IVF | 0.59 | 0.95 | 33.5 | |
| Farm VIIIF | 0.28 | 1.47 | 9.6 | |
| Chelex® Method 3 | PEK F | 0.51 | 1.04 | 164.35 |
| Farm IVF | 0.50 | 1.04 | 136.45 | |
| Farm VIF | 0.42 | 1.02 | 81.15 | |
| Bead-beating Tube Method | Farm IF | 0.34 | 1.05 | 63 |
| Farm I Centrifuged | 0.58 | 1.09 | 14.25 |
F: Fill-FLOTAC Solution; DT: Direct Faecal Sample. PEK: Positive control sample.
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