Table 3
Optimisation for the tagged LAMP amplicon concentration for the LF assay.
| LF Dipstick ID | Loading Sample and Kit Buffer Volumes |
|---|---|
| RX1a | 1 μL amplicon + 100 μL buffer |
| RX1b | 0.25 μL amplicon + 100 μL buffer |
| RX1c | (1 μL amplicon + 9 μL molecular grade water) + 100 μL buffer |
| NTCa | 1 μL amplicon + 100 μL buffer |
| NTCb | 0.25 μL amplicon + 100 μL buffer |
| NTCb | (1 μL amplicon + 9 μL molecular grade water) + 100 μL buffer |
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