Table 3

Optimisation for the tagged LAMP amplicon concentration for the LF assay.

LF Dipstick ID Loading Sample and Kit Buffer Volumes
RX1a 1 μL amplicon + 100 μL buffer
RX1b 0.25 μL amplicon + 100 μL buffer
RX1c (1 μL amplicon + 9 μL molecular grade water) + 100 μL buffer
NTCa 1 μL amplicon + 100 μL buffer
NTCb 0.25 μL amplicon + 100 μL buffer
NTCb (1 μL amplicon + 9 μL molecular grade water) + 100 μL buffer

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