Table 1
Average EPG of the farms (n > 25 sheep per farm) used in the respective assay designs.
Farm ID | Average EPG | Result of Assay Used |
Remarks | |||
---|---|---|---|---|---|---|
LAMP Optimisation | LF Assay Optimisation | Optimised LAMP | PCR | |||
Farm I | 600 | ✓ | NA | ✓ | ✓ | |
Farm II | 150* | *✖ | NA | ✓ | ✓ | *✖: pooled exclusive of samples with suspected trichostrongyle eggs |
Farm III | 276.47 | ✓ | NA | ✓ | ✓ | |
Farm IV | 982.22 | NA | ✓ | ✓ | ✓ | |
Farm V | 1750 | NA | ✓ | ✓ | ✓ | |
Farm VI | 5.76 | NA | ✖ | ✖ | ✖ | |
Farm VII | 113.70 | NA | ✓ | ✓ | ✓ | |
Farm VIII | 767.20 | NA | ✓ | ✓ | ✓ | |
Farm IX | 168 | NA | ✓ | ✓ | ✓ | |
Farm X | – | NA | NA | ✓ | ✓ | |
Farm DF** | – | ✓ | NA | ✓ | ✓ | |
Farm GF*** | – | ½ | NA | ✓ | ✓ | ½: One weak positive |
*Only three individual sheep had trichostrongyle egg counts. **Adult worms only. *** Pooled faecal sample submitted by the farmer. NA: Not applicable as it was not used during the optimisation. ✓: Positive result. ✖: Negative result.
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