Fig. 4


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LF-LAMP assay. Optimised assay tested against three random farm samples. A: LAMP assay using tagged primers. Yellow/Orange = Positive/Weak Positive, Pink/Purple = Negative. B: LF-LAMP assay using the tagged LAMP amplicons. Positive detection of H. contortus presents as a band in both Control and Test lines. Negative detection presents as a band in only the Control line. Failed assay presents as no bands on LF strip. 1: Farm V. 2: Farm VIII. 3: Farm X. NTC: Non-template control. PC: Positive control gDNA of H. contortus.

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