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Research Article

Table 3

Review of studies comparing the performances of PCR and microscopy for helminth detection.

Targets Micro +
Micro +
Proportion of false-negative from PCR Reference
PCR + PCR −
Ascaris spp. 7 1 13% This study
154 7 4% [17]
34a 2a 6%a [13]
23 5 18% [20]
8 0 0% This study (+bb)
35a 1a 3%a [13] (+bb)
192 27 12% [15] (+bb)
E. vermicularisb 7 4 36% This study
8 3 27% This study (+bb)
Hookworms 6 7 54% This study
89 9 9% [17]
48 1 2% [10]
136 15 10% [15] (+bb)
Hymenolepis spp.b 20 1 5% This study
21 0 0% This study (+bb)
Strongyloides spp.c 28 7 20% This study
30 5 14% This study (+bb)
33 21 39% [25]
88 12 12% [17]
14 14 50% [5]
Taenia spp. 5 0 0% This study
7 0 0% [18]
T. trichiura 1 10 91% This study
18a 9a 33%a [13]
10 1 9% This study (+bb)
26a 1a 4%a [13] (+bb)
297 23 7% [3] (+bb)

Micro: Microscopy; (+bb): with bead-beating pretreatment.

a

In this study, different conditions were evaluated for sample preservation and pretreatment. In order to be in conditions similar to our study, the results shown in this table correspond to frozen samples without preservative.

b

No published evaluation compared PCR and microscopy for E. vermicularis and Hymenolepis spp.

c

As many evaluations of S. stercoralis have been published, sometimes with substantial bias, we focused on studies comparing real-time PCR targeting the 18S rRNA gene to a combination of parasitological methods as the reference, and without age restriction of the population.

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