Evaluation of the Allplex™ GI-Helminth(I) Assay, the first marketed multiplex PCR for helminth diagnosis

Open Access

Table 3

Review of studies comparing the performances of PCR and microscopy for helminth detection.

Targets	Micro +	Micro +	Proportion of false-negative from PCR	Reference
Targets	PCR +	PCR −	Proportion of false-negative from PCR	Reference
Ascaris spp.	7	1	13%	This study
	154	7	4%	[17]
	34^a	2^a	6%^a	[13]
	23	5	18%	[20]
	8	0	0%	This study (+bb)
	35^a	1^a	3%^a	[13] (+bb)
	192	27	12%	[15] (+bb)

E. vermicularis^b	7	4	36%	This study
E. vermicularis^b	8	3	27%	This study (+bb)

Hookworms	6	7	54%	This study
	89	9	9%	[17]
	48	1	2%	[10]
	136	15	10%	[15] (+bb)

Hymenolepis spp.^b	20	1	5%	This study
Hymenolepis spp.^b	21	0	0%	This study (+bb)

Strongyloides spp.^c	28	7	20%	This study
	30	5	14%	This study (+bb)
	33	21	39%	[25]
	88	12	12%	[17]
	14	14	50%	[5]

Taenia spp.	5	0	0%	This study
Taenia spp.	7	0	0%	[18]

T. trichiura	1	10	91%	This study
	18^a	9^a	33%^a	[13]
	10	1	9%	This study (+bb)
	26^a	1^a	4%^a	[13] (+bb)
	297	23	7%	[3] (+bb)

Micro: Microscopy; (+bb): with bead-beating pretreatment.

In this study, different conditions were evaluated for sample preservation and pretreatment. In order to be in conditions similar to our study, the results shown in this table correspond to frozen samples without preservative.

No published evaluation compared PCR and microscopy for E. vermicularis and Hymenolepis spp.

As many evaluations of S. stercoralis have been published, sometimes with substantial bias, we focused on studies comparing real-time PCR targeting the 18S rRNA gene to a combination of parasitological methods as the reference, and without age restriction of the population.

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