Evaluation of the Allplex™ GI-Helminth(I) Assay, the first marketed multiplex PCR for helminth diagnosis

Open Access

Table 1

Comparative results of Allplex™ GI-Helminth(I) Assay and microscopy (N = 110 samples).

Targets	Number of samples with				Concordance: % (CI95)	Cohen’s kappa: κ (CI95)
	Micro +	Micro −	Micro +	Micro −
	GIPH +	GIPH −	GIPH −	GIPH +
Hookworms^a	6	97	7	0	94% (87; 97)	0.60 (0.34; 0.86)
A. duodenale	2	108	0	0	100% (97; 100)	1.00 (1.00; 1.00)
N. americanus	4	104	2	0	98% (94; 100)	0.79 (0.51; 1.00)
Ascaris spp.	7	102	1	0	99% (95; 100)	0.93 (0.79; 1.00)
E. vermicularis	7	99	4	0	96% (92; 99)	0.76 (0.53; 0.98)
Hymenolepis spp.	20	87	1	2	97% (91; 99)	0.91 (0.82; 1.00)
Strongyloides spp.	28	73	7	2	92% (85; 96)	0.80 (0.68; 0.93)
Taenia spp.	5	105	0	0	100% (97; 100)	1.00 (1.00; 1.00)
T. trichiura	1	99	10	0	91% (84; 96)	0.15 (−0.11; 0.42)
All targets	67	10	30^b	4^b	70% (61; 78)	0.24 (0.08; 0.40)

Micro: Microscopy; GIPH: Allplex™ GI – Helminth(I) assay.

As egg morphology does not allow species-level identification of hookworms, this row combines the results for both A. duodenale and N. americanus PCRs. The rows below consider only samples with positive culture, which allowed species-level identification.

One sample was both “Microscopy +/GIPH −” for A. lumbricoides and “Microscopy −/GIPH +” for S. stercoralis, and was therefore counted in each column but considered as a single sample for the calculations.

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