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Table 1

Comparative results of Allplex™ GI-Helminth(I) Assay and microscopy (N = 110 samples).

Targets Number of samples with
Concordance: % (CI95) Cohen’s kappa: κ (CI95)
Micro +
Micro −
Micro +
Micro −
GIPH + GIPH − GIPH − GIPH +
Hookwormsa 6 97 7 0 94% (87; 97) 0.60 (0.34; 0.86)
 A. duodenale 2 108 0 0 100% (97; 100) 1.00 (1.00; 1.00)
 N. americanus 4 104 2 0 98% (94; 100) 0.79 (0.51; 1.00)
Ascaris spp. 7 102 1 0 99% (95; 100) 0.93 (0.79; 1.00)
E. vermicularis 7 99 4 0 96% (92; 99) 0.76 (0.53; 0.98)
Hymenolepis spp. 20 87 1 2 97% (91; 99) 0.91 (0.82; 1.00)
Strongyloides spp. 28 73 7 2 92% (85; 96) 0.80 (0.68; 0.93)
Taenia spp. 5 105 0 0 100% (97; 100) 1.00 (1.00; 1.00)
T. trichiura 1 99 10 0 91% (84; 96) 0.15 (−0.11; 0.42)
All targets 67 10 30b 4b 70% (61; 78) 0.24 (0.08; 0.40)

Micro: Microscopy; GIPH: Allplex™ GI – Helminth(I) assay.

a

As egg morphology does not allow species-level identification of hookworms, this row combines the results for both A. duodenale and N. americanus PCRs. The rows below consider only samples with positive culture, which allowed species-level identification.

b

One sample was both “Microscopy +/GIPH −” for A. lumbricoides and “Microscopy −/GIPH +” for S. stercoralis, and was therefore counted in each column but considered as a single sample for the calculations.

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