Table 3
Inhibition of infection of macrophages. The inhibition of infection of murine macrophages using pre-treated parasites was performed by incubating L. infantum stationary promastigotes (5 × 106 cells) with b-AD (0, 3.09, 6.19 and 12.39 μM) or AmpB (0, 0.27, 0.54 and 1.08 μM) for 4 h at 24 °C. Parasites were then washed in RPMI 1640 and used to infect murine cells at a ratio of 10 parasites per macrophage, for 48 h at 37 °C in 5% CO2. The percentage of infected macrophages, the infectiveness reduction, and the number of amastigotes per treated macrophage were evaluated by counting 200 macrophages in triplicate. Results are shown as mean ± standard deviation of the groups.
| Compound | Concentration (μM) | Infection percentage using pre-treated parasites | Infectiveness reduction (%) | Number of amastigotes per macrophage |
|---|---|---|---|---|
| β-acetyl-digitoxin | 12.39 | 8.8 ± 2.0 | 84.8 | 0.2 ± 0.1 |
| 6.19 | 13.3 ± 2.4 | 77.0 | 0.7 ± 0.3 | |
| 3.09 | 27.4 ± 4.0 | 52.6 | 1.6 ± 0.5 | |
| 0 | 57.8 ± 5.2 | (–) | 3.3 ± 0.6 | |
| Amphotericin B | 1.08 | 18.4 ± 2.6 | 68.2 | 0.7 ± 0.3 |
| 0.54 | 26.5 ± 2.7 | 54.2 | 1.2 ± 0.3 | |
| 0.27 | 38.7 ± 4.3 | 33.0 | 2.2 ± 0.4 | |
| 0 | 57.8 ± 5.2 | (–) | 3.3 ± 0.6 |
Current usage metrics show cumulative count of Article Views (full-text article views including HTML views, PDF and ePub downloads, according to the available data) and Abstracts Views on Vision4Press platform.
Data correspond to usage on the plateform after 2015. The current usage metrics is available 48-96 hours after online publication and is updated daily on week days.
Initial download of the metrics may take a while.