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Research Article

Table 2

Evaluation of treatment of infected macrophages. Murine macrophages (5 × 105 cells) were cultured in complete RPMI 1640 medium for 24 h at 37 °C in 5% CO2. After, cells were washed twice with PBS and L. infantum stationary promastigotes were added to the cultures at a ratio of 10 parasites per macrophage, for 48 h at 37 °C in 5% CO2. Free parasites were removed by washing with RPMI 1640 medium, and infected macrophages were treated with b-AD (0, 3.09, 6.19 and 12.39 μM) or AmpB (0, 0.27, 0.54 and 1.08 μM) for 48 h at 24 °C in 5% CO2. The percentage of infected cells, the infectiveness reduction, and the number of recovered amastigotes per cell were determined by counting 200 macrophages in triplicate. Results are shown as mean ± standard deviation of the groups.

Compound Concentration (μM) Percentage of infected macrophages after treatment Infectiveness reduction (%) Number of amastigotes per macrophage
β-acetyl-digitoxin 12.39 7.6 ± 1.2 89.0 0.1 ± 0
6.19 14.3 ± 2.5 79.2 0.4 ± 0.2
3.09 24.3 ± 3.3 64.6 1.2 ± 0.4
0 68.7 ± 4.0 (–) 3.9 ± 0.5
Amphotericin B 1.08 19.8 ± 2.4 71.2 0.8 ± 0.4
0.54 32.1 ± 3.8 53.3 1.3 ± 0.3
0.27 42.5 ± 5.2 38.1 2.1 ± 0.4
0 68.7 ± 4.0 (–) 3.9 ± 0.5

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