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Investigation of intracytoplasmic cytokine-producing T cell frequency. Cytometry flow was performed to evaluate the IFN-γ, TNF-α and IL-10-producing T CD4+ and CD8+ cell frequency in splenocytes of mice treated, 15 days post-therapy. Results were calculated by the ratio between SLA-stimulated versus unstimulated cultures (SLA/CC ratio), and reported as cytokine indexes for CD4+ and CD8+ T cells. Bars represent the mean plus standard deviation of the groups. (*) indicates a significant difference as compared to the saline and B/Mic groups (p < 0.05). (**) indicates a statistically significant difference as compared to the miltefosine group (p < 0.05). (***) indicates a statistically significant difference as compared to the b-AD group (p < 0.05).
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