T-cell subtypes involved in the IFN-γ production. Splenocytes of mice treated with miltefosine, b-AD or b-AD/Mic (n = 6 per group) were cultured in DMEM (medium) or stimulated with SLA (50.0 μg/mL) in the presence of anti-CD4 or anti-CD8 antibody, for 48 h at 37 °C in 5% CO₂. IFN-γ levels were quantified in the cell supernatant by a capture ELISA. Bars represent the mean ± standard deviation of the groups. (*) indicates a statistically significant difference as compared to the use of anti-CD4 or anti-CD8 antibody (p < 0.05). (**) indicates a statistically significant difference as compared to the use of anti-CD8 antibody (p < 0.05).