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Organ parasitism evaluated in the treated animals by a limiting dilution technique. Mice (n = 12 per group) were infected with L. infantum and, 1 and 15 days after the distinct treatment schedules, they (n = 6 per group in each time) were euthanized and their livers, spleens, bone marrow (BM) and draining lymph nodes (dLNs) were collected to evaluate the parasite load, by a limiting dilution technique. Bars represent the mean ± standard deviation of the groups. (*) indicates a significant difference as compared to the saline and B/Mic groups (p < 0.05). (**) indicates a statistically significant difference as compared to the miltefosine group (p < 0.05). (***) indicates a statistically significant difference as compared to the b-AD group (p < 0.05).
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