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Phylogenetic tree based on neighbor-joining (N-J) analysis of ITS sequences. Phylogenetic relationships between the E. bieneusi genotypes identified in cattle here and other known genotypes deposited in GenBank were inferred by an N-J analysis of ITS sequences based on genetic distance by the Kimura two-parameter model. The numbers on the branches are percent bootstrapping values from 1000 replicates. Each sequence is identified by its accession number, host origin, and genotype designation. Enterocytozoon bieneusi genotype CSK2 (KY706128) was used as the outgroup. The squares and triangles filled in black indicate novel and known genotypes identified in this study, respectively.

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