Issue |
Parasite
Volume 27, 2020
|
|
---|---|---|
Article Number | 65 | |
Number of page(s) | 8 | |
DOI | https://doi.org/10.1051/parasite/2020065 | |
Published online | 24 November 2020 |
Research Article
Genotyping and zoonotic potential of Enterocytozoon bieneusi in cattle farmed in Hainan Province, the southernmost region of China
Génotypage et potentiel zoonotique d’Enterocytozoon bieneusi chez les bovins élevés dans la province de Hainan, la région la plus au sud de la Chine
1
Institute of Animal Science and Veterinary Medicine, Hainan Academy of Agricultural Sciences, 571100 Haikou, PR China
2
Key Laboratory of Tropical Translational Medicine of the Ministry of Education, Hainan Medical University, 571199 Haikou, PR China
3
Department of Pathogenic Biology, Hainan Medical University, 571199 Haikou, Hainan, PR China
4
Hainan Medical University-The University of Hong Kong Joint Laboratory of Tropical Infectious Diseases, Hainan Medical University, 571199 Haikou, Hainan, PR China
* Corresponding authors: chaozhe.cn@126.com; luganghn@163.com
Received:
7
October
2020
Accepted:
9
November
2020
Enterocytozoon bieneusi is an intestinal pathogen that infects a wide range of species, including humans. Cattle constitute an important host for E. bieneusi; however, there is a scarcity of information on the prevalence and genotyping of E. bieneusi in cattle in the Hainan Province of China. In this study, PCR analysis of 314 fecal samples from cattle in six cities of Hainan was performed for genotype identification. The average prevalence of E. bieneusi in these animals was 9.9% (31/314), and ranged from 0.0% (0/12) to 20.5% (8/39). Five known genotypes – EbpC (n = 14), BEB4 (n = 12), J (n = 2), I (n = 1), and CHG5 (n = 1) – and a novel genotype: HNC-I (n = 1) – were identified. Genotypes EbpC and HNC-I were placed in zoonotic Group 1, and the remaining four genotypes (BEB4, J, I, and CHG5) were placed in Group 2. Since 93.5% of the genotypes found in the cattle (29/31) (EbpC, BEB4, J, and I) have previously been found in humans, these genotypes are probably involved in the transmission of microsporidiosis to humans.
Résumé
Enterocytozoon bieneusi est un pathogène intestinal qui infecte un large éventail d’espèces, y compris les humains. Le bétail constitue un hôte important pour E. bieneusi, mais les informations sur la prévalence et le génotypage d’E. bieneusi chez les bovins de la province de Hainan en Chine sont rares. Dans cette étude, une analyse PCR de 314 échantillons fécaux provenant de bovins dans six villes de Hainan a été réalisée pour l’identification du génotype. La prévalence moyenne d’E. bieneusi chez ces animaux était de 9,9 % (31/314), et variait de 0,0 % (0/12) à 20,5 % (8/39). Cinq génotypes connus, EbpC (n = 14), BEB4 (n = 12), J (n = 2), I (n = 1) et CHG5 (n = 1), et un nouveau génotype, HNC-I (n = 1), ont été identifiés. Les génotypes EbpC et HNC-I sont placés dans le groupe zoonotique 1, et les quatre génotypes restants (BEB4, J, I et CHG5) sont placés dans le groupe 2. Puisque 93,5 % (29/31) (EbpC, BEB4, J et I) des génotypes trouvés chez les bovins ont déjà été trouvés chez l’homme, ces génotypes sont probablement impliqués dans la transmission de la microsporidiose à l’homme.
Key words: Enterocytozoon bieneusi / Cattle / Genotyping / Hainan (China)
© X.-L. Zheng et al., published by EDP Sciences, 2020
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