Open Access

Table 1

Prevalence of Cryptosporidium infections in lambs and ewes determined by molecular analysis.

Farms Animals Number examined Animals positive for Cryptosporidium species
Cryptosporidium parvum genotyping
18S rRNA gene-based PCR Lib13 locus and 18S rRNA sequence analysis
#1 Lambs 20 13 (65%) 13 ND
Ewes 18 3 (16.7%) 2 C. parvum * 1 C. parvum IIdA21G2
1 ND 1 C. parvum IIdA15G1
#2 Lambs 8 4 (50%) 1 C. xiaoi
3 ND
Ewes 9 3 (33.3%) 1 C. parvum * 1 ND
1 C. ubiquitum
1 ND
#3 Lambs 18 14 (77.8%) 2 C. parvum * 2 C. parvum IIdA24G1
12 ND
Ewes 10 5 (50%) 1 C. xiaoi
4 ND
#4 Lambs 13 0
Ewes 16 2 (12.5%) 1 C. parvum * 1 C. parvum IIaA16G3R1
1 ND
#5 Lambs 20 5 (25%) 2 C. parvum * 2 ND
3 ND
Ewes 19 2 (10.5%) 2 C. parvum * 1 C. parvum IIaA16G3R1
1 C. parvum IIaA13G2R1
Total Lambs 79 36 (45.6%) 4 C. parvum * 2 C. parvum IIdA24G1
1 C. xiaoi
31 ND
Ewes 72 17 (23.6%) 6 C. parvum * 2 C. parvum IIaA16G3R1
3 C. xiaoi 1 C. parvum IIdA21G2
1 C. ubiquitum 1 C. parvum IIdA15G1
7 ND 1 C. parvum IIaA13G2R1

ND: not determined due to sequencing failure.

*

Determined by Lib13 PCR result.

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