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DNA immunization augmented the frequency of antigen-specific T cells in mice. (A) Total numbers of CD3+ CD4+ CD8− T lymphocytes per spleen. (B) Total numbers of CD3+ CD8+ CD4− T lymphocytes per spleen. Data are means ± SD (representative of three experiments). *p < 0.05, **p < 0.01, and ***p < 0.001, compared with the control groups.

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