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Research Article

Table 2

Screening for dirofilariosis in a military kennel in the Indre department (central France).

Dog number (No.) Breed* Age (year) Kennel presence time (year) Parasitological diagnosis: Knott test Serological screening
Molecular detection of filarial DNA using the qPCR Pan-Filaria 28S Genotyping: 18S rRNA gene Molecular identification of filarial species using a COI Triplex qPCR-based system
Molecular identification of Wolbachia genotypes using ftsZ duplex qPCR-based system
Diagnosis
Witness® Dirofilaria DiroCHEK® Dirofilaria immitis Dirofilaria repens Acanthocheilonema reconditum Wolbachia endosymbiont of D. immitis Wolbachia endosymbiont of D. repens
1 BSM 10 8.5 Neg. Neg. Neg. Neg. NE** Neg. Neg. Neg. Neg. Neg. Healthy dog
2 BSM 7 6 D. repens Pos. Pos. Pos. D. repens Neg. Pos. Neg. Pos. Pos. Occult heartworm and subcutaneous dirofilariosis
3 GS 6 5 D. immitis Pos. Pos. Pos. D. immitis Pos. Neg. Neg. Pos. Neg. Heartworm disease
4 BSM 6 5 Neg. Neg. Neg. Neg. NE Neg. Neg. Neg. Neg. Neg. Healthy dog
5 BSM 6 5 Neg. Neg. Neg. Neg. NE Neg. Neg. Neg. Neg. Neg. Healthy dog
6 BSM 6.5 5 Neg. Neg. Neg. Neg. NE Neg. Neg. Neg. Neg. Neg. Healthy dog
7 GS 6 4 D. immitis + D. repens Neg. Pos. Pos. NE Pos. Pos. Neg. Pos. Pos. Heartworm and subcutaneous dirofilariosis
8 BSM 4.5 3 Neg. Neg. Neg. Pos. NE Pos. Pos. Neg. Pos. Pos. Occult heartworm and subcutaneous dirofilariosis
9 GS 3.5 2.5 Neg. Neg. Neg. Neg. NE Neg. Neg. Neg. Neg. Neg. Healthy dog
10 BSM 4 2 D. repens Neg. Neg. Pos. D. repens Neg. Pos. Neg. Pos. Pos. Occult heartworm and subcutaneous dirofilariosis
11 BSM 3 2 Neg. Neg. Neg. Neg. NE Neg. Neg. Neg. Neg. Neg. Healthy dog
12 GS 3 2 Neg. Neg. Neg. Neg. NE Neg. Neg. Neg. Neg. Neg. Healthy dog
13 GS 4 1.5 D. repens Pos. Pos. Pos. D. repens Neg. Pos. Neg. Pos. Pos. Occult heartworm and subcutaneous dirofilariosis
14 GS 2.5 1.5 Neg. Neg. Neg. Neg. NE Neg. Neg. Neg. Neg. Neg. Healthy dog
15 BSM 3.5 1 Neg. Neg. Neg. Neg. NE Neg. Neg. Neg. Neg. Neg. Healthy dog
16 BSM 2 0.5 Neg. Neg. Neg. Neg. NE Neg. Neg. Neg. Neg. Neg. Healthy dog
17 BSM 2 0.5 Neg. Neg. Neg. Neg. NE Neg. Neg. Neg. Neg. Neg. Healthy dog
*

BSM: Belgian shepherd malinois, GS: German shepherd.

**

NE: Not evaluated.

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