Open Access
Table 1
Primer sequences of target genes coding for antigenic proteins, used in this study.
| PCR end-point |
Real-time PCR |
|||||
|---|---|---|---|---|---|---|
| Gene locus | Sequences (5′ – 3′) | Ta/°C | Size (bp) | Sequences (5′ – 3′) | Ta/°C | Size (bp) |
| A. peg-1 | F: ATCCTCTTCACATTCGCTTT | 57 °C | 639 | F: CATGTGCCGATAAATGCGGG | 57 °C | 130 |
| R: GGATAATAATGGTCGGGCAA | R: CCCTGTGAGCATGCATCCTT | |||||
| A. peg-7 | F: ACACCTCCATCTGAACAAA | 57 °C | 899 | F: TATCGGAATGCGTGACTGCA | 57 °C | 130 |
| R: CCTAACATGCAGGCGATTA | R: AGGCAGTTTCCATGGTGTATG | |||||
| A. peg-13 | F: CATGAAATCACTCGAACACG | 55 °C | 931 | F: AAACATTCGACGCCTACACC | 60 °C | 108 |
| R: TGTTCCTCCTTGTGCTCT | R: CATCGTGGTCTTCTCTGCGA | |||||
| GAPDH | – | – | – | F: CCCCTTCATCAACATCGACT | 60 °C | 152 |
| R: TCAGCTCCCCATTTGATTTC | ||||||
GAPDH, glyceraldehyde-3-phosphate dehydrogenase (housekeeping gene); PCR, polymerase chain reaction.
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