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Table 1

Primer sequences of target genes coding for antigenic proteins, used in this study.

PCR end-point
Real-time PCR
Gene locus Sequences (5′ – 3′) Ta/°C Size (bp) Sequences (5′ – 3′) Ta/°C Size (bp)
A. peg-1 F: ATCCTCTTCACATTCGCTTT 57 °C 639 F: CATGTGCCGATAAATGCGGG 57 °C 130
R: GGATAATAATGGTCGGGCAA R: CCCTGTGAGCATGCATCCTT
A. peg-7 F: ACACCTCCATCTGAACAAA 57 °C 899 F: TATCGGAATGCGTGACTGCA 57 °C 130
R: CCTAACATGCAGGCGATTA R: AGGCAGTTTCCATGGTGTATG
A. peg-13 F: CATGAAATCACTCGAACACG 55 °C 931 F: AAACATTCGACGCCTACACC 60 °C 108
R: TGTTCCTCCTTGTGCTCT R: CATCGTGGTCTTCTCTGCGA
GAPDH F: CCCCTTCATCAACATCGACT 60 °C 152
R: TCAGCTCCCCATTTGATTTC

GAPDH, glyceraldehyde-3-phosphate dehydrogenase (housekeeping gene); PCR, polymerase chain reaction.

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