Relative expression profiles of the gene coding for the ESPs proteins, A.peg-1, A.peg-7, A.peg-13 (shown vertically on the right side of the graph), normalised to the geometric mean of GAPDH (gpd2), in A. pegreffii larvae, in response to temperature conditions (7 °C, 20 °C and 37 °C, after 24 h). The control at 2 °C is shown as normalised to a value of 1. Each value represents the M ± SD of three biological replicates. Significant differential expression was assessed by Student’s t test. Significance was fixed at p < 0.05 [*p < 0.05, **p < 0.01, ***p < 0.001, p < 0.0001].