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Nucleotide and amino acid sequence alignment of A.peg-7 cDNA. (a) Nucleotide sequence alignment obtained from A. pegreffii larvae from the present study with respect to the internal Ani s 7 cDNA (nucleotide [nt] 1203–2139) fragment [a potential antigenic region] of those previously deposited in GenBank as A. simplex (s. l.) (Accession No.: EF158010). Dots indicate identity with the consensus sequence. (b) Amino acid sequence alignment of the deduced amino acid sequences of the A.peg-7 allergen showing the variable sites between A. pegreffii, and previously reported A. simplex (s. l.). Dots represent residues identical to the reference Ani s 7 sequence.

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