Coenzyme Q2 is a universal substrate for the measurement of respiratory chain enzyme activities in trypanosomatids

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Table 1

Respiratory chain enzyme activities with different substrates.

NADH dehydrogenase		Activity [U/mg]	SD	Inh. DPI %	Inh. Rot. %	Cytochrome c reductase		Activity [mU/mg]	SD	Inh. Ant. %
P. serpens	Q₁	0	0	–	–	T. brucei (PF)	Q₁H	432	98	27
	Q₂	53	25	37	36		Q₂H	383	124	86
	DB	45	4	0	17		DBH	410	101	95
T. brucei (PF)	Q₁	65	23	100	–	L. tarentolae	Q₁H	609	19	19
	Q₂	81	19	74	–		Q₂H	611	118	83
	DB	43	29	2	–		DBH	755	151	96

Succinate dehydrogenase		Activity [U/mg]	SD	Inh. Mal. %		Alternative oxidase		Activity [U/mg]	SD	Inh. SHAM %
P. serpens	Q₁	20	11	94		P. serpens	Q₁H	398	120	89
	Q₂	24	10	100			Q₂H	538	71	79
	DB	19	4	100			DBH	105	5	92
T. brucei (PF)	Q₁	45	12	99		T. brucei (PF)	Q₁H	8	3	7
	Q₂	54	14	98			Q₂H	10	4	84
	DB	27	12	100			DBH	0	0	–
L. tarentolae	Q₁	104	28	99		T. brucei (BF)	Q₁H	146	51	84
	Q₂	122	18	99			Q₂H	282	83	95
	DB	86	11	100			DBH	52	1	0

Average values of enzyme activities are displayed in Figure 2 where activities [U] of individual enzymes are also defined; SD – standard deviations; Inh. – the rate of inhibition with the corresponding inhibitor in %; DPI – diphenyl iodonium (150 μM); Rot. – rotenone (10 μM); Ant. – Antimycin A (150 μM); Mal. – sodium malonate (1 mM); SHAM – salicylhydroxamic acid (10 μM); Inhibition with rotenone was not measured with T. brucei, because it was already shown previously that NADH dehydrogenase activity in this organism is not sensitive to rotenone [23].

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