Open Access
Table 1
Primers used in the PCR assays conducted in this study with their respective references.
| Organism | Targeted gene (product size) | Name and primer sequences (5′ – 3′) | PCR conditions | Ref. |
|---|---|---|---|---|
| Tick | Mitochondrial 16S rDNA (320 bp) | TQ 16S+1F: | Denaturation | [2] |
| 5′-CTGCTCAATGATTTTTTAAATTGCTGTGG-3′ | 94 °C 5 min | |||
| TQ 16S−2R: | Hybridization | |||
| 5′-ACGCTGTTATCCCTAGAG-3′ | 10 cycles: 92 °C 1 min; 48 °C 1 min; 72 °C 1 min 30 s | |||
| 32 cycles: 92 °C 1 min; 54 °C 1 min; 72 °C 1 min 30 s | ||||
| Extension | ||||
| 72 °C 5 min | ||||
| Anaplasmataceae | 16S rRNA (345 bp) | EHR 16SD: | Denaturation | [36] |
| 5′-GGTACCYACAGAAGAAGTCC-3′ | 94 °C 5 min | |||
| EHR 16SR: | Hybridization | |||
| 5′-TAGCACTCATCGTTTACAGC-3′ | 34 cycles: 94 °C 40 s; 50 °C 40 s; 72 °C 1 min | |||
| Extension | ||||
| 72 °C 10 min | ||||
| Anaplasma phagocytophilum | 16S rDNA (932 bp) | ge3a: | Denaturation | [28] |
| 5′-CACATGCAAGTCGAACGGATTATTC-3′ | 95 °C 5 min | |||
| ge10r: | Hybridization | |||
| 5′-TTCCGTTAAGAAGGATCTAATCTCC-3′ | 40 cycles: 94 °C 30 s; 55 °C 30 s; 72 °C 1 min | |||
| Extension | ||||
| 72 °C 5 min | ||||
| 16S rDNA (546 bp) | ge9f: | Denaturation | ||
| 5′-AACGGATTATTCTTTATAGCTTGCT-3′ | 95 °C 5 min | |||
| ge2r: | Hybridization | |||
| 5′-GGCAGTATTAAAAGCAGCTCCAGG-3′ | 30 cycles: 94 °C 30 s; 55 °C 30 s; 72 °C 1 min | |||
| Extension | ||||
| 72 °C 5 min | ||||
| Borrelia burgdorferi s.l. | 16S rRNA (357 bp) | 16S LDF: | Denaturation | [27] |
| 5′-ATGCACACTTGGTGTTAACTA-3′ | 95 °C 5 min | |||
| 16S LDR: | Hybridization | |||
| 5′-GACTTATCACCGGCAGTCTTA-3′ | 35 cycles: 95 °C 1 min; 53 °C 1 min; 72 °C 1 min | |||
| Extension | ||||
| 72 °C 10 min | ||||
| Babesia/Theileria spp. | 18S rRNA (359 bp) | BAB GF2: | Denaturation | [4] |
| 5′-GYYTTGTAATTGGAATGATGG-3′ | 94 °C 5 min | |||
| BAB GR2: | Hybridization | |||
| 5′-CCAAAGACTTTGATTTCTCTC-3′ | 35 cycles: 94 °C 1 min; 60 °C 1 min; 72 °C 1 min | |||
| Extension | ||||
| 72 °C 10 min | ||||
| Rickettsia spp. | gltA (citrate synthase) (381 bp) | RpCS.877p: | Denaturation | [39] |
| 5′-GGGGGCCTGCTCACGGCGG-3′ | 95 °C 5 min | |||
| RpCS.1258n: | Hybridization | |||
| 5′-ATTGCAAAAAGTACAGTGAACA-3′ | 6 cycles: 94 °C 1 min; 60 °C 1 min; 72 °C 1 min | |||
| 30 cycles: 94 °C 1 min; 53 °C 1 min; 72 °C 1 min | ||||
| Extension | ||||
| 72 °C 10 min | ||||
| SFG Rickettsia | ompA (532 bp) | Rr190.70p: | Denaturation | [39] |
| 5′-ATGGCGAATATTTCTCCAAAA-3′ | 95 °C 5 min | |||
| Rr190.602n: | Hybridization | |||
| 5′-AGTGCAGCATTCGCTCCCCCT-3′ | 6 cycles : 94 °C 1 min; 60 °C 1 min; 72 °C 1 min | |||
| 30 cycles : 94 °C 1 min; 53 °C 1 min; 72 °C 1 min | ||||
| Extension | ||||
| 72 °C 10 min |
Current usage metrics show cumulative count of Article Views (full-text article views including HTML views, PDF and ePub downloads, according to the available data) and Abstracts Views on Vision4Press platform.
Data correspond to usage on the plateform after 2015. The current usage metrics is available 48-96 hours after online publication and is updated daily on week days.
Initial download of the metrics may take a while.