iTRAQ-based comparative proteomic analysis of cells infected with Eimeria tenella sporozoites

Open Access

Table 2

Fold changes in the mRNA expression of 12 genes differently expressed in BHK cells infected with Eimeria tenella, detected by qPCR.

		Ratio				Ratio
Accession	Gene name	iBR1	iBR2	iBR3	Average	qPCR1	qPCR2	qPCR3	Average
A0A061ILM3	Atp51	2.940	2.786	2.879	1.63	1.04	1.05	1.09	1.06
A0A1A6H7C7	Mapk9	1.382	1.388	1.374	1.38	1.98	2.13	2.13	2.08
G3HED1	Ptp4a1^*	1.300	1.358	1.273	1.31^*	0.24	0.36	0.30	0.30^*
G3GRN2	Bnip2^*	3.290	3.328	3.740	3.45^*	0.60	0.68	0.82	0.70^*
A0A061ILM3	Pdss2	2.940	2.786	2.879	2.87	1.38	1.49	1.69	1.52
G3II02	Ppp2r4	3.580	3.441	3.427	3.48	1.58	1.96	1.59	1.71
A0A061HU28	Gdi1	0.827	0.657	0.724	0.74	0.36	0.50	0.40	0.42
G3HIC4	Sec61a1	0.782	0.733	0.769	0.76	0.38	0.56	0.44	0.46
G3GRA0	Tm7sf2	0.796	0.705	0.697	0.73	0.08	0.06	0.10	0.08
A0A1A6GC37	Rps8	0.706	0.755	0.824	0.76	0.50	0.55	0.48	0.51
G3I429	Mrp120	0.771	0.797	0.731	0.76	0.58	0.66	0.80	0.68
A0A060BA20	Tfrc	0.722	0.830	0.740	0.76	0.56	0.74	0.80	0.70

Expression patterns detected by qRT-PCR were not consistent with those obtained by iTRAQ.

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