Levels of cytokines in splenocyte culture. Splenocytes were obtained from immunized and control mice prior to the challenge and cultivated in 96-well plates. The levels of IFN-γ, IL-2, IL-4, IL-10, and IL-12p70 cytokines in splenic culture supernatants were determined using ELISA. No significant difference was detected between pVAX1-HSP40-immunized mice and control mouse groups (p > 0.05).