MALDI-TOF MS spectra of Anopheles gambiae Giles and Anopheles coluzzii abdomen protein extracts engorged on host blood vertebrates. All mosquitoes were collected only at times 1 and 24 h post-feeding. Alignment of MS spectra from: H1_An_coluzzii_Camelus dromedarius (A), H24_An_coluzzii_ Camelus dromedarius (B), H1_ An_coluzzii Ammotragus lervia (C), H24_ An_coluzzii Ammotragus lervia(D), H1_ An_coluzzii _Sus scrofa (E), H24_ An_coluzzii _Sus scrofa (F), H1_ An_gambiae _Papio hamadryas (G), H24_ An_gambiae _Papio hamadryas (H), H1_An_gambiae_ Antidorcas marsupialus (I), H24_An_gambiae_ Antidorcas marsupialus (J), H1_An_gambiae_Arctictis binturong (K), H24_An_gambiae_ Arctictis binturong (L), H1_ An_gambiae_ _Panthera onca (M), H24_ An_gambiae_ _Panthera onca (N), H1_An_gambiae_Leopardus pardalis (O), and H24_An_gambiae_ Leopardus pardalis (P). a.u. arbitrary units; m/z mass-to-charge ratio.