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LM images of B. grimi. A. Specimens fixed in formalin (5%) and soaked in glycerine-alcohol (10%), showing the oval body shape, vestibulum (vb) and macronucleus (ma), a round contractile vacuole (cv) in the posterior and a cytoproct (cp) at the end of the body. Scale bar = 10 µm. B. Specimens fixed in formalin (5%) and soaked in glycerine-alcohol (10%), showing the long vestibulum (vb) surrounded by cilia. Scale bar = 10 µm. C-F. are protargol stained: C. showing the body shape, macronucleus (ma) and micronucleus (mi). Scale bar = 10 µm. D. showing the vestibulum and somatic kineties. Scale bar = 10 µm. E. showing the vestibulum (vb) and the oval macronucleus (ma) with a spherical micronucleus (mi) embedded in the middle. Scale bar = 10 µm. F. showing the relative position of macronucleus (ma) and micronucleus (mi). Scale bar = 5 µm.
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