Open Access
Table 1
Primers for T. gondii and Anaplasma detection in goats and PCR amplification conditions.
| Pathogens | Methods | Primer | Amplicon (bp) |
Thermocycler program | Cycles | Final extension | Reference | |||
|---|---|---|---|---|---|---|---|---|---|---|
| Initial denaturation | Cycle | |||||||||
| A. ovis | PCR | 5′-CCGGATCCTTAGCTGAACAGGAATCTTGC-3′ 5′-GGGAGCTCCTATGAATTACAGAGAATTGTTTAC-3′ |
867 | 94°C 30 s |
94°C 30 s |
60°C 30 s |
58°C 1 min |
30 | 72°C 5 min |
[9] |
| A. bovis | PCR | 5’-TCCTGGCTCAGAACGAACGCTGGCGGC-3’ 5’-AGTCACTGACCCAACCTTAAATGGCTG-3’ |
1433 | 94°C 5 min |
94°C 30 s |
55°C 30 s |
72°C 30 s |
30 | 72°C 10 min |
[3] |
| nPCR* | 5′-CTCGTAGCTTGCTATGAGAAC-3′ 5′-TCTCCCGGACTCCAGTCTG-3′ |
551 | 94°C 5 min |
94°C 30 s |
55°C 30 s |
72°C 30 s |
30 | 72°C 10 min |
[13] | |
| A. phagocytophilum | PCR | 5’-TCCTGGCTCAGAACGAACGCTGGCGGC-3’ 5’-AGTCACTGACCCAACCTTAAATGGCTG-3’ |
1433 | 94°C 5 min |
94°C 30 s |
55°C 30 s |
72°C 30 s |
30 | 72°C 10 min |
[3] |
| nPCR | 5′-GCTGAATGTGGGGATAATTTAT-3′ 5′-ATGGCTGCTTCCTTTCGGTTA-3′ |
641 | 94°C 5 min |
94°C 30 s |
55°C 30 s |
72°C 30 s |
30 | 72°C 10 min |
[13] | |
| T. gondii | PCR | 5’-CCGCGGAGCCGAAGTG −3’ 5’-TAGATCGCATTCCGGTGTCTC-3’ |
287 | 94°C 5 min |
94°C 30 s |
55°C 30 s |
72°C 30 s |
35 | 72°C 10 min |
[17] |
| nPCR | 5’-GGACAGAAGTCGAAGGGGAC-3’ 5’-TTCCACCCTGCAGGAAAAGC −3’ |
181 | 94°C 5 min |
94°C 30 s |
55°C 30 s |
72°C 30 s |
30 | 72°C 5 min |
[17] | |
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