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Multiple sequences alignment from T. gondii aminopeptidase N (M1 peptidase family) and several selected members of the M1 family of zinc-metallopeptidases: P. falciparum (PF3D7_1311800 and PF3D7_1472400), T. gondii (TGME49_221310, TGME49_224350, and TGME49_224460), N caninum (NCLIV_048240 and NCLIV_048230), and C. parvum (cgd8_3430). Amino acid positions identical between these sequences and the T. gondii sequence are in darkened letters. Identical (black background) and conserved (grey background) amino acids between all sequences are indicated. The position of the conserved putative zinc ion ligands (L), the conserved glutamyl residue required for catalytic activity (C), and the conserved putative proton donor (D) are indicated in bold on the bottom line. The amino acid numbers for each sequence are indicated on the left. The position of gaps is indicated by full colons. Alignments were performed using the ClustalW2 algorithm ( with the Blosum 62 matrix.

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