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Table 1

 Multiplex PCR fragment sizes of the 12 taxa of the genus Trichinella.

Primer set Locusa Trichinella taxon fragment size (bp)
Tsp Tna/Tpat Tbr/T8/T9 Tps Tmu T6 Tne Tpa Tzi
I ESV 173 127 127 310–360 127 127 155 240 264
II ITS1 253 256
III ITS1 210
IV ITS2 316
V ITS2 404

Trichinella spiralis(Tsp), T. nativa(Tna), T. britovi(Tbr), T. pseudospiralis(Tps), T. murrelli(Tmu), T. nelsoni(Tne), T. papuae(Tpa), T. zimbabwenzis(Tzi), T. patagoniensis(Tpat) and Trichinella genotypesT6, T8 and T9. The size (bp) of the ITS1 fragment of T. murrelli is in bold. The fragment sizes are those from Zarlenga et al. (1999) [24], Pozio and La Rosa (2010) [20] and Krivokapich et al. (2012) [13].

a

ESV, expansion segment V region of the ribosomal DNA repeat; ITS1 and ITS2, internal transcribed spacers 1 and 2.

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