Open Access
Research Article
Table 1
Primers and Probes used in this study.
| Gene | Sequence |
|---|---|
| GAPDH | Forward primer: 5′ −CAAGCCCATCACCATCTTCCA- 3′ |
| Reverse primer: 5′ −CGGTGGACTCCACAACATACTC- 3′ | |
| Probe: 5′ −FAM-CCGCCAACATCAAATG-MGB- 3′ | |
| IL-4 | Forward primer: 5′ −CAGGGTGCTCCGCAAATTT- 3′ |
| Reverse primer: 5′ −GACCCCGGAGTTGTTCTTCA- 3′ | |
| Probe: 5′ −FAM-ACTTCCCACGAGAGGTG-MGB- 3′ | |
| IL-6 | Forward primer: 5′ −AGGATCCAGGTCAAATAGTCTTTCC- 3′ |
| Reverse primer: 5′ −TTCCGTCTGTGACTCCAGTTTCT- 3′ | |
| Probe: 5′ −FAM-CCCAACTTCCGAGGCG-MGB- 3′ | |
| IL-10 | Forward primer: 5′ −CAAGGCAGCCTTGCAGAAG- 3′ |
| Reverse primer: 5′ −TCCAGCCAGTAAGATTAGGCAATA- 3′ | |
| Probe: 5′ −FAM-CTCCATCATGCCCAGCT-MGB- 3′ | |
| IFN-γ | Forward primer: 5′ −TTGGGCCCTCTGACTTCGT- 3′ |
| Reverse primer: 5′ −CAGTGTGTAGCGTTCATGGTCTCT- 3′ | |
| Probe: 5′ −FAM-CCGGACTTGCCCTGC-MGB- 3′ | |
| TNF | Forward primer: 5′ −CACTCAGGTCCTCTTCTCAGAAC- 3′ |
| Reverse primer: 5′ −TGGTGGTTGGGTACGACATG- 3′ | |
| Probe: 5′ −FAM-CCAGCGACAAGCCTG-MGB- 3′ | |
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