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Research Article

Table 1

Primers and Probes used in this study.

Gene Sequence
GAPDH Forward primer: 5′ −CAAGCCCATCACCATCTTCCA- 3′
  Reverse primer: 5′ −CGGTGGACTCCACAACATACTC- 3′
  Probe: 5′ −FAM-CCGCCAACATCAAATG-MGB- 3′
IL-4 Forward primer: 5′ −CAGGGTGCTCCGCAAATTT- 3′
  Reverse primer: 5′ −GACCCCGGAGTTGTTCTTCA- 3′
  Probe: 5′ −FAM-ACTTCCCACGAGAGGTG-MGB- 3′
IL-6 Forward primer: 5′ −AGGATCCAGGTCAAATAGTCTTTCC- 3′
  Reverse primer: 5′ −TTCCGTCTGTGACTCCAGTTTCT- 3′
  Probe: 5′ −FAM-CCCAACTTCCGAGGCG-MGB- 3′
IL-10 Forward primer: 5′ −CAAGGCAGCCTTGCAGAAG- 3′
  Reverse primer: 5′ −TCCAGCCAGTAAGATTAGGCAATA- 3′
  Probe: 5′ −FAM-CTCCATCATGCCCAGCT-MGB- 3′
IFN-γ Forward primer: 5′ −TTGGGCCCTCTGACTTCGT- 3′
  Reverse primer: 5′ −CAGTGTGTAGCGTTCATGGTCTCT- 3′
  Probe: 5′ −FAM-CCGGACTTGCCCTGC-MGB- 3′
TNF Forward primer: 5′ −CACTCAGGTCCTCTTCTCAGAAC- 3′
  Reverse primer: 5′ −TGGTGGTTGGGTACGACATG- 3′
  Probe: 5′ −FAM-CCAGCGACAAGCCTG-MGB- 3′

FAM: 6-carboxyfluorescein; MGB: Minor Groove Binder.

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