Quantitation of cytokine mRNA in the spleen, brain and blood. Gerbils were euthanized under anesthesia at days 1, 3, 5, 7, 9 and 11 after intraperitoneal inoculation with PbA. mRNA levels were measured by reverse transcription (RT)-PCR, and values were expressed as mean (±S.E.M., N = 5) fold changes compared with values from control, uninfected gerbils. a. IL-4 mRNA expression. b. IL-10 mRNA expression. c. IL-6 mRNA expression. d. IFN mRNA expression. e. TNF mRNA expression. All data are representative of two independent experiments and compared by one-way analysis of variance (ANOVA) with Tukey's multiple comparison post-hoc tests for differences between groups.