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Table 1.

Primers and hydrolysis probes used to detect Echinococcus multilocularis, Toxoplasma gondii and Toxocara spp. parasites, and carnivore host identification of copro-samples.

Targeted species Gene Sequence name Nucleotide sequence Reference
Echinococcus multilocularis rrnL rrn-Fwd 5′-CTGTGATCTTGGTGTAGTAGTTGAGATTT3′ [25]
rrn-Rev 5′-GGCTTACGCCGGTCTTAACTC-3′
rrn-probe 5′-TGGTCTGTTCGACCTTTTTAGCCTCCAT-3′
Toxocara spp. Cox1 Toxo-Fwd 5′-AAAATAGCCAAATCCACACTACTACCA-3′ [26]
Toxo-Rev 5′-GGTGTGGGACTAGTTGAACTGTGTA-3′
Toxo-probe 5′-CCCCATAGTCCTCAAAG-3′
Toxoplasma gondii 529-rep Tg-Fwd 5′-AGAGACACCGGAATGCGATCT-3′ [32]
Tg-Rev 5′-CCCTCTTCTCCACTCTTCAATTCT-3′
Tg-probe 5′-ACGCTTTCCTCGTGGTGATGGCG-3′
Vulpes vulpes CytB Vv-Fwd 5′-ACCTTCCCGCACCATCAAA-3′ [26]
Vv-Rev 5′-TGTTGCAATCTGTAGAATAAGGCATA-3′
Vv-probe 5′-CTGCCTGATGGAACTTCGGGTCCC-3′
Canis l. familiaris CytB Cf-Fwd 5′-CCACCCACTAGCCAAAATTGTT-3′ [26]
Cc-Rev 5′-AAGTTCCATCAAGCAGAGATGTTAGA-3′
Cf-probe 5′-ATAACTCATTCATTGACCTCCCAGCGCC
Felis s. catus CytB Fc-Fwd 5′-CCCTTCTAGGAGTCTGCCTAATCTT-3′ [26]
Fc-Rev 5′-CGGTTATTGTGTCTGATGTGTAGTGT-3′
Fc-probe 5′-AAATCCTCACCGGCCTCTTTTTGGC-3′
Geotrichum candidum CytB Geo-Fwd 5′-CACCGCCCGTCGCTAC-3′ [25]
Geo-Rev 5′-AGAAAAGTTGCCCTCTCCAGTT-3′
  Geo-probe 5′-TCAATCCGGAAGCCTCACTAAGCCATT-3′

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