Open Access
Table 1
Overview of the target gene, primer and PCR methods used for pathogen identification in sampled ticks.
| Pathogen | Target gene | Primer sequence (5'–3') | Method | Reference |
|---|---|---|---|---|
| Ehrlichia canis/ Anaplasma platys | 16S rRNA gene | Outer primer F: AGAGTTTGATCCTGGCTCAG Outer primer R: TAGCACTCATCGTTTACAGC Nested Primer: A. platys-specific primers F: AAGTCGAACGGATTTTGTC, and Primer R: CTTTAACTTACCGAACC E.canis- specific primers F: CAATTATTTATAGCCTCTGGCTATAGGA, and Primer R: GAGTTTGCCGGGACTTCTTCT |
Nested PCR | [33] |
| Babesia gibsoni/ Babesia canis | 18S rRNA gene | PIRO-A: AGGGAGCCTGAGAGACGGCTACC PIRO-B: TTAAATACGAATGCCCCCAAC |
PCR | [34] |
| Borrelia burgdorferi sensu lato | Flagellin gene | Outer primer F: TGGTATGGGAGTTTCTGG Outer primer R: TCTGTCATTGTAGCATCTTT Nested primer F: CAGACAACAGAGGGAAAT Nested primer R:TCAAGTCTATTTTGGAAAGCACC |
Nested PCR | [35] |
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