IFN-γ production of spleen lymphocyte cultures from immunized mice.
|Groupa||IFN-γ (fold change)b
|PLG-rSAG1/2 MPs (single I.P. dose)||5.3 ± 1.6c||2.6 ± 0.7e|
|rSAG1/2 (Vet L-10) (single I.P. dose)||1.0 ± 0.4d||2.4 ± 0.3e|
|rSAG1/2 (Vet L-10) (two I.P. doses)||1.2 ± 0.2d||2.5 ± 0.4e|
|rSAG1/2 (single I.P. dose)||1.1 ± 0.3d||2.3 ± 0.6e|
|PLG (single I.P. dose)||1.0 ± 0.1d||2.1 ± 0.4e|
|PBS (single I.P. dose)||0.9 ± 0.2d||2.1 ± 0.2e|
Twelve weeks after immunization, spleen lymphocytes isolated from different groups of mice were stimulated with 5 μg/mL of TsoAg or 1 μg/mL of Con A for 6 h at 37 °C. Total cellular RNA was then extracted for the assay of IFN-γ production by real-time PCR.
The fold change was determined by dividing the quantity of specific IFN-γ mRNA from stimulated cells by the quantity of IFN-γ mRNA from the unstimulated cells.
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