Protective immunity against toxoplasmosis in mice induced by single-dose immunization with rSAG1/2 protein released from poly(lactide-co-glycolide) microparticles

Open Access

Table 1.

IFN-γ production of spleen lymphocyte cultures from immunized mice.

Group^a	IFN-γ (fold change)^b
	TsoAg	Con A
PLG-rSAG1/2 MPs (single I.P. dose)	5.3 ± 1.6^c	2.6 ± 0.7^e
rSAG1/2 (Vet L-10) (single I.P. dose)	1.0 ± 0.4^d	2.4 ± 0.3^e
rSAG1/2 (Vet L-10) (two I.P. doses)	1.2 ± 0.2^d	2.5 ± 0.4^e
rSAG1/2 (single I.P. dose)	1.1 ± 0.3^d	2.3 ± 0.6^e
PLG (single I.P. dose)	1.0 ± 0.1^d	2.1 ± 0.4^e
PBS (single I.P. dose)	0.9 ± 0.2^d	2.1 ± 0.2^e

Twelve weeks after immunization, spleen lymphocytes isolated from different groups of mice were stimulated with 5 μg/mL of TsoAg or 1 μg/mL of Con A for 6 h at 37 °C. Total cellular RNA was then extracted for the assay of IFN-γ production by real-time PCR.

The fold change was determined by dividing the quantity of specific IFN-γ mRNA from stimulated cells by the quantity of IFN-γ mRNA from the unstimulated cells.

^c–e

A significant difference (P < 0.05) exists between groups with different superscript letters.

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