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Research Article

Table 1.

IFN-γ production of spleen lymphocyte cultures from immunized mice.

Groupa IFN-γ (fold change)b
TsoAg Con A
PLG-rSAG1/2 MPs (single I.P. dose) 5.3 ± 1.6c 2.6 ± 0.7e
rSAG1/2 (Vet L-10) (single I.P. dose) 1.0 ± 0.4d 2.4 ± 0.3e
rSAG1/2 (Vet L-10) (two I.P. doses) 1.2 ± 0.2d 2.5 ± 0.4e
rSAG1/2 (single I.P. dose) 1.1 ± 0.3d 2.3 ± 0.6e
PLG (single I.P. dose) 1.0 ± 0.1d 2.1 ± 0.4e
PBS (single I.P. dose) 0.9 ± 0.2d 2.1 ± 0.2e
a

Twelve weeks after immunization, spleen lymphocytes isolated from different groups of mice were stimulated with 5 μg/mL of TsoAg or 1 μg/mL of Con A for 6 h at 37 °C. Total cellular RNA was then extracted for the assay of IFN-γ production by real-time PCR.

b

The fold change was determined by dividing the quantity of specific IFN-γ mRNA from stimulated cells by the quantity of IFN-γ mRNA from the unstimulated cells.

c–e

A significant difference (P < 0.05) exists between groups with different superscript letters.

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