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Table 2.
Statistical measures of the performance of ELISA (OD) and MAT with different cut-offs compared with MC-PCR for detecting T. gondii in horse meat samples.
| MC-PCR* vs. OD > 0.06 | MC-PCR* vs. OD > 0.1015 | MC-PCR* vs. OD > 0.1145 | MC-PCR* vs. OD > 0.15 | MC-PCR* vs. MAT > 0 | MC-PCR* vs. MAT > 1:10 | MC-PCR* vs. MAT > 1:20 | MC-PCR* vs. MAT > 1:40 | |
|---|---|---|---|---|---|---|---|---|
| Sensitivity, % | 90.91% | 65.66% | 61.62% | 49.49% | 65.66% | 45.45% | 32.32% | 19.19% |
| (95% CI) | (0.85–0.97) | (0.56–0.75) | (0.52–0.71) | (0.40–0.59) | (0.56–0.75) | (0.36–0.55) | (0.23–0.42) | (0.11–0.27) |
| Specificity, % | 11.36% | 46.97% | 48.48% | 57.58% | 46.21% | 70.45% | 84.09% | 92.42% |
| (95% CI) | (0.06–0.17) | (0.38–0.55) | (0.40–0.57) | (0.49–0.66) | (0.38–0.55) | (0.63–0.78) | (0.78–0.90) | (0.88–0.97) |
| PPV, % | 43.48% | 48.15% | 47.29% | 46.67% | 47.79% | 53.57% | 60.38% | 65.52% |
| (95% CI) | (0.37–0.50) | (0.40–0.57) | (0.39–0.56) | (0.37–0.56) | (0.39–0.56) | (0.43–0.64) | (0.47–0.74) | (0.48–0.83) |
| NPV, % | 62.50% | 64.58% | 62.75% | 60.32% | 64.21% | 63.27% | 62.36% | 60.40% |
| (95% CI) | (0.43–0.82) | (0.55–0.74) | (0.53–0.72) | (0.52–0.69) | (0.55–0.74) | (0.55–0.71) | (0.55–0.69) | (0.54–0.67) |
| Kappa coefficient | 0.02 | 0.12 | 0.09 | 0.07 | 0.11 | 0.16 | 0.17 | 0.12 |
| McNemar test | P < 0.1% | P < 0.1% | P < 1% | NS | P < 0.1% | NS | P < 0.1% | P < 0.1% |
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