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Table 3.

Production costs for 100 metacercariae in Galba truncatula and Pseudosuccinea columella infected with Fasciola hepatica (five miracidia per snail at exposure).

Parameters P. columella G. truncatula (controls)
Time (hrs) spent by a technician
 Exposure of snails to miracidia and preparation of Petri dishes 4.0 4.0
 Surveillance of breeding recipients for snails 9.5 15.5
 Count of metacercariae every three days during the patent period 28.5 21.0
 Transfer of metacercariae to Eppendorf tubes by aspiration 0.33 0.33
 Total 42.33 40.83
Cost of time (Euros) spent by a technician* 969.3 935.0
Purchase price of materials used for 100 snails (Euros)
 Consumables 7.51 7.51
 Lettuce 4.12 2.88
Total cost for 100 snails (Euros) 980.9 945.3
Number of snails at miracidial exposure 200 200
Number of metacercariae encysted on dish walls and bottom 25,856: Beni Suef 12,136: Migné,
18,065: Castelmoron 8,687: Thenay
Cost price for 100 metacercariae in Euros (US dollars) per snail exposed to miracidia** 3.79 (4.16): Beni Suef 7.78 (8.55): Migné
5.42 (5.96): Castelmoron 10.88 (11.96): Thenay
4.60 (5.06): Both 9.33 (10.26): Both
*

Cost price (estimated) for a technician: 1 h = 22.9 Euros.

**

1 Euro = 1.10 US dollars.

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