Open Access

Table 2.

Neff’s Amoeba Saline (AS) [71]. 10 mL of each stock solution (10×) are added to 950 mL dH2O, mixed, sterilized by filtration and aliquoted into needed volumes.

Stocks (10×) Grams per 100 mL ddH2O
NaCl 1.20
MgSO4-7H2O 0.04
CaCl2 · 2H2O 0.04
Na2HPO4 1.42
KH2PO4 1.36

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