Neff’s Amoeba Saline (AS) . 10 mL of each stock solution (10×) are added to 950 mL dH2O, mixed, sterilized by filtration and aliquoted into needed volumes.
|Stocks (10×)||Grams per 100 mL ddH2O|
|CaCl2 · 2H2O||0.04|
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