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Table 3.

Microscopy measurements of hemogregarine intracellular parasites and infected host erythrocytes under 1000× magnification. An extracellular gametocyte was also detected for the sample ACZC1827 but was not included in these measurements (19.22 µm in length and 2.26 µm in width, similar to mean measurements for free gamonts in previous studies [38]). n refers to the number of hemogregarine gamonts or infected host cells measured per sample.

Host species Code Hemogregarines – Mean ± SD (min − max)
Host cell – Mean ± SD (min − max)
n Vertical Horizontal Area Perimeter n Vertical Horizontal Area Perimeter
Furcifer sp. md67 10 12.89 ± 0.94 (11.09 − 14.14) 4.31 ± 0.71 (2.95 − 5.10) 49.67 ± 5.36 (39.19 − 56.40) 34.78 ± 2.00 (31.23 − 38.36) 10 17.84 ± 1.45 (15.19 − 19.89) 11.02 ± 0.87 (9.97 − 13.01) 157.67 ± 11.48 (137.15 − 173.51) 51.74 ± 2.00 (48.77 − 54.79)
Ithycyphus oursi ACZC1932 5 14.49 ± 0.46 (13.95 − 15.28) 3.77 ± 0.86 (2.56 − 5.21) 58.64 ± 9.21 (45.28 − 72.23) 39.12 ± 1.07 (37.59 − 40.66) 5 18.29 ± 1.35 (16.32 − 19.58) 10.82 ± 1.53 (8.13 − 12.76) 163.41 ± 31.87 (112.91 − 208.07) 52.84 ± 4.98 (45.46 − 58.57)
Madagascarophis colubrinus ACZC1827 10 13.82 ± 0.56 (12.55 − 14.61) 2.97 ± 0.55 (1.83 − 3.59) 42.77 ± 8.73 (24.78 − 57.26) 36.43 ± 2.13 (32.60 − 40.81) 8 19.93 ± 1.59 (16.32 − 22.08) 11.20 ± 1.01 (9.97 − 13.20) 180.91 ± 10.63 (164.88 − 194.65) 56.80 ± 1.23 (55.10 − 58.87)
13.73 ± 0.66 (11.09 − 15.28) 3.68 ± 0.71 (1.83 − 5.21) 50.36 ± 7.77 (24.78 − 72.23) 36.78 ± 1.73 (31.23 − 40.81)
Madagascarophis colubrinus ACZC1963* 10 11.53 ± 1.16 (10.12 − 14.40) 3.42 ± 0.35 (2.94 − 4.02) 35.93 ± 4.07 (30.85 − 42.80) 30.76 ± 2.33 (27.95 − 36.59) 9 15.99 ± 2.20 (12.54 − 19.30) 9.72 ± 1.41 (8.24 − 12.76) 123.34 ± 13.97 (92.00 − 142.14) 46.35 ± 3.42 (40.00 − 52.88)
Oplorus sp. ×49** 10 12.13 ± 0.51 (11.25 − 13.19) 5.88 ± 0.67 (4.72 − 7.01) 59.71 ± 5.90 (48.30 − 66.92) 34.26 ± 1.24 (32.38 − 36.08) 6 14.61 ± 1.21 (12.33 − 15.80) 10.54 ± 1.19 (8.24 − 11.84) 124.23 ± 15.90 (96.91 − 141.63) 45.15 ± 2.78 (41.68 − 49.10)
*

PCR was negative for sample ACZC 1963.

**

Sample ×49 presented morphologically distinct parasites (see Fig. 1), some of which apparently found inside leukocytes and yielded a genetically distinct 18S rRNA gene haplotype (Fig. 3). Samples md67, ACZC1932 and ACZC1827 yielded identical 18S rRNA gene sequences (Fig. 3), so a mean is presented for these as they may correspond to the same Hepatozoon species (Hepatozoon domerguei, see Discussion and Fig. 3). GenBank accession numbers are given in Figure 3.

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