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Western blot analysis of native proteins in three developmental stages. The expression of the native proteins encoded by the three var3 genes (PF3D7_0100300, PF3D7_0600400, and PF3D7_0937600) in the ring, trophozoite, and schizont stages were, respectively, detected by the variant-specific antibodies generated in rats. Lanes 1 and 2 represent results from infected red blood cells and uninfected red blood cells, respectively. A band of approximately 152 kDa was detected in the late trophozoite stage (24–30 h p.i.) with the antibody specific to PF3D7_0937600. A band of approximately 153 kDa was detected in the schizont stage (36–44 h p.i.) with the antibody specific to PF3D7_0100300. And a band of approximately 70 kDa was detected in both the trophozoite stage and schizont stage (24–44 h p.i.) for the PF3D7_0600400-specific antibody.
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