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Table 2.

Main characteristics of F. hepatica infection in snails maintained to a constant temperature of 20 °C (controls), or subjected every week to a 3-h stay at a mean temperature of 12 °C (TC, temperature-challenged; CS, cercariae-shedding; SD, standard deviation).

Parameters Population (A)
Population (B)
Controls TC snails Controls TC snails
Number of snails at day 30 p.e. 81 81 77 78
Number (frequency in %):
 CS snails 23 (28.3) 49 (60.4)* 14 (18.1) 37 (47.4)*
 NCS snails 35 (43.2) 6 (7.4) 24 (31.1) 9 (11.5)
Prevalence of infection (%) 71.6 67.9 49.3 58.9
Mean growth (SD) of CS snails during experiment (mm) 3.2 (0.8) 3.1 (1.0) 2.3 (0.7) 2.3 (0.9)
Mean length (SD) in days:
 Prepatent period 49.5 (7.7) 48.3 (3.0) 50.2 (8.3) 48.2 (3.1)
 Patent period 30.7 (9.2) 46.2 (10.3)* 24.5 (11.5) 38.7 (8.9)*
Mean number (SD) of mcc shed by CS snails
 Floating mcc 11.5 (5.3) 13.3 (4.8) 7.2 (3.1) 4.5 (1.7)
 Fixed and complete mcc 217.1 (69.3) 304.0 (80.5)* 134.6 (50.4) 214.0 (71.0)*
 Fixed and incomplete mcc 3.2 (2.4) 44.3 (27.2)* 1.5 (0.7) 30.6 (11.4)*
Total mcc production:
 Per CS snail 231.8 361.6 143.3 249.1
 Per snail at day 30 p.e. 58.5 218.7 26.0 118.1
*

Significant differences between temperature-challenged snails and controls.

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