Microfilariae injected in the pleural cavity of CBA/Ca mice did not reach peripheral blood and accumulate in the lungs.

A: after inoculation of 250,000 microfilariae into the pleural cavity of BALB/c (white square) and CBA/Ca (black square) microfilaremia was monitored on blood smear over 24 days. Results are presented as mean ± SEM (p < 0.0001); B: microfilarial density in the pleural cavity 24 hours p.i. and 24 days p.i. was evaluated on pleural wash smear. Arbitrary score was set up: +++ > 50,000, ++ > 10,000, + > 1,000; C. parasitic load in the lungs, spleen and liver 24 hours p.i. of CBA/Ca and BALB/c mice was detected by quantitative amplification of COI. Results are expressed as the median + range of six observations for each tissue and represented as a stacked barplot.