Volume 19, Number 3, August 2012
|Page(s)||277 - 280|
|Published online||15 August 2012|
Prosorhynchus crucibulum (Digenea: Bucephalidae) miracidium morphology and its passive transmission pattern
Morphologie et mode de transmission passive du miracidium de Prosorhynchus crucibulum (Digenea: Bucephalidae)
Universidade do Porto, Faculdade de Ciências, Departamento de Biologia, Rua do Campo Alegre, s/n., FC4, 4169-007 Porto, Portugal, and CIMAR Laboratório Associado / CIIMAR, Centro de Investigação Marinha e Ambiental, Rua dos Bragas, 289, 4050-123 Porto, Portugal
* Correspondence: Maria João Santos. Tel.: 351 220 402 805 – Fax: 351 220 402 709. E-mail: email@example.com
Accepted: 7 May 2012
The characterization of Prosorhynchus crucibulum (Rudolphi, 1819) Odhner, 1905 egg and miracidium is important in order to better understand the transmission dynamics between the definitive host and the primary host, the mussel. In this way, the objective of this work was to study the miracidia morphology, in order to evaluate if this species belongs to the group of passive or active transmission larvae. The morphology of eggs is similar to the ones presented by other Prosorhynchus species, with a small size of 26 × 17 μm, and four-five times smaller than the ones of Fasciola hepatica. The number of eggs produced per worm was around 6,760 (4,236-8,401), which was four-five times higher than in F. hepatica. The miracidia presented small dimensions 24 × 15 μm (23-25 × 13-15 μm range), a long stylet, two ciliated epithelial plates, very long cilia (12.7 μm) and absence of terebratorium and eyespots. Those features of the miracidia suggest that P. crucibulum belongs to the group of passively infecting larvae.
La morphologie des oeufs et du miracidium de Prosorhynchus crucibulum (Rudolphi, 1819) Odhner, 1905 est importante afin de mieux comprendre le mode de transmission entre l’hôte définitif et l’hôte intermédiaire, la moule. L’objectif de ce travail était d’étudier la morphologie du miracidium afin de savoir si l’espèce appartient au groupe des larves à transmission passive ou active. La morphologie des oeufs est semblable à celle d’autres espèces de Prosorhynchus, avec une taille de 26 × 17 μm, quatre-cinq fois plus petite que celle de Fasciola hepatica. Le nombre moyen d’oeufs produits par ver est de 6 760 (4 236-8 401), quatre-cinq fois plus élevé que celui observé avec F. hepatica. Le miracidium est également de petite dimension, 24 × 15 μm (23-25 × 13-15 μm), avec un long stylet, les deux plans épithéliaux ciliés, des cils de dimension très longue (12,7 μm) et l’absence de terebratorium et d’ocelle. Ces caractéristiques du miracidium de P. crucibulum suggèrent que l’espèce appartient au groupe des larves à infection passive.
Key words: Prosorhynchus crucibulum / egg / miracidia / transmission / morphology
Mots clés : Prosorhynchus crucibulum / oeuf / miracidium / transmission / morphologie
© PRINCEPS Editions, Paris, 2012, transferred to Société Française de Parasitologie
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
The mussel (Mytilus spp.), a highly appreciated mollusc and therefore an important commercial species in southern Europe, is the first intermediate host of the bucephalid digenean Prosorhynchus crucibulum (Rudolphi, 1819) Odhner, 1905 (Matthews 1973, Cousteau et al., 1990; McGladdery et al., 1999). Moreover, Prosorhynchus sp. infection had been described as causing serious problems in mussel, like castration and weakening of the adductor muscle (Cousteau et al., 1990; Cousteau et al., 1993; Shelley et al., 1988; Lasiak, 1992; Calvo & Mcquaid, 1998; Silva et al., 2002; Cochôa & Magalhães, 2008; Francisco et al., 2010). Although there are some studies on the ecology, biology and morphology characteristics of adult and metacercariae of the genus Prosorhynchus Odhner, 1905 (Jones, 1943; Matthews, 1973; Santos & Gibson, 2002; Laffargue et al., 2004; Etchegoin et al., 2005; Bray & Justine, 2006), information about the egg or miracidium stages and it life cycle dynamics are scantily presented. Recently, the miracidium active way of infection in Bucephalidae was questioned (Galaktionov & Dobrovolskij, 2003). Therefore, the main aim of this work was to characterize the transmission of egg and/or miracidium P. crucibulum to the first host, and characterize the larva morphology comparing with Fasciola hepatica in order to classify them as passive or active miracidium.
Adult worms of P. crucibulum (n = 14) were collected from four freshly caught conger eels (Conger conger), its definitive host. First, we used P. crucibulum (n = 8), to estimate average number of eggs per adult worm. The number of eggs per adult from F. hepatica (n = 2) was also counted for comparison purposes.
The study of P. crucibulum egg and miracidium morphology was performed by light microscopy (LM), and the observations were made with a Zeiss Axiophot microscope, equipped with a digital camera Zeiss Axiocam Icc3 and image analysis software (AxioVision 4.6). The eggs were placed in a small drop of saline water (35 ‰ salinity) on a slide and analysed. The live miracidia morphology was studied from eggs that were artificially hatched, by pressing them with a cover glass; some were observed fresh while others were later stained in methylene blue or eosin. The miracidium morphology of F. hepatica, an active infective larvae, was redrawn here for comparison purposes (Fig. 1) with our species.
The estimated average number of eggs per adult worm (n = 8) in P. crucibulum was 6,760 (4,236-8,401). The percentage and average number ± standard deviation (range) of immature and mature eggs in the adult worms was 21%, 1,396 (281- 2,233) and 79%, 5,363 (3,331-7,278), respectively. For comparison with our values we determined the same variables in F. hepatica. The minimum number of eggs (n = 2) in adult worms of F. hepatica and its average number was 1,459 ± 730 (296-1,163), presenting a ratio of young and mature eggs of 16% [114 ± 59 (72-156)] and 84% [616 ± 554 (224-1,007)].
The eggs from P. crucibulum (n = 10) presented 26 × 17 µm in average size and 24-27 × 11-20 µm in range (Table I). The shell coloration of the eggs varied between transparent for the immature eggs and greenchestnut for the mature eggs.
Measurements of Prosorhynchus spp. eggs.
The miracidium from P. crucibulum (Fig. 2) measured 24 × 15 µm (23-25 × 13-15 µm range), around five times shorter than F. hepatica. The cilia covered the whole surface of the body, arranged in two-row of epithelial plates, and not in several plates as in F. hepatica. A long cilia with 12.7 (11.8-13.7) µm in length. Stylet located outside of the apical gland. Terebratorium and eyespots absent. Four germinal cells and eight nucleus of somatic cells were also observed.
Drawing of a miracidium of Prosorhynchus crucibulum (Rudolphi, 1819) Odner, 1905, observed with light microscopy, covered with peripheral cilia and showing two epithelial plates (arrows) in the body.
In the Atlantic coast, two mussel species can serve as first intermediate host to P. crucibulum, they are Mytilus edulis and M. galloprovincialis (Matthews, 1973; Cousteau et al., 1990; Teia dos Santos & Coimbra, 1995; McGladdery et al., 1999). Therefore, to study the trematode strategy for reaching the first intermediate host, it is relevant to understand the dynamics of its life cycle. P. crucibulum life cycle was studied by Matthews (1973), who also observed different stages of egg development (immature and mature) within the uterus of P. crucibulum, what is corroborated by the findings reported in our work. The percentage of each development stage was similar in P. crucibulum and F. hepatica. However, the minimum number of eggs/worm was different in both species, being higher in P. crucibulum than in F. hepatica, besides their different adult size (the former are 4-5 times smaller than the later). With regard to the dimensions of the eggs recorded here, we can see that they are similar to the ones recorded in other Prosorhynchus species, such as: P. aculeatus, P. maternus, P. pacificus, P. atlanticus and P. australis (Jones, 1943; Winter, 1960; Etchegoin et al., 2005; Bray & Justine, 2006). However, they are not similar to the ones found in F. hepatica that are 4-5 times bigger (Duwel, 1982).
Therefore, we can note that P. crucibulum and F. hepatica have different strategies of egg production; the former has small eggs, and smaller miracidia, in large number, and the later has large eggs and larger miracidia in small number. This could be related to different strategies to achieve the first intermediate host, the mollusc.
The two strategies of the miracidium that are currently recognized in the literature are: some larvae have an active way of infection, while others have a passive way. According to Galaktionov & Dobrovolskij (2003), these are associated with different morphologies of the larva. F. hepatica miracidium, which is an active infecting larva, presents a large size, an apical papilla, several ciliated epithelial plates and eyespots. While, in P. crucibulum miracidium we have reported several features that belong to the second group: small size, only two ciliated epithelial plates, terebratorium absent, stylet present and situated outside the apical gland and eyespots absent. In summary, we can say that the morphology of the miracidium from P. crucibulum is very simplified compared with that of F. hepatica. The same pattern was also recorded for P. squamatus and was associated to its passive way of infection (Galaktionov & Dobrovolskij, 2003). The active infection of the first host for bucephalids, generally accepted, was questioned by those authors and is here confirmed by the reported features that they most probably have a passive way of infection.
This study was supported by CAPES (grant no 37787-053) to C.J. Francisco, and to M.J. Santos (Project FCOMP-01-0124-FEDER-020726 / FCT-PTDC/ MAR/116838/2010).
- Bray A.R. & Justine J.L. Prosorhynchus maternus sp. n. (Digenea: Bucephalidae) from the Malabar grouper Epinephelus malabaricus (Perciformes: Serranidae) of New Caledonia. Folia Parasitologica, 2006, 53, 181–188. [PubMed] [Google Scholar]
- Cousteau C., Combes C., Maillard C., Renaud F. & Delay B. Prosorhynchus squamatus (Trematoda) Parasitosis in the Mytilus edulis-Mytilus galloprovincialis complex: specificity and host-parasite relationships. Pathology in Marine Science, 1990, 291–298. [Google Scholar]
- Cousteau C., Robbins I., Delay B., Renaud F. & Mathieus M. The parasitic castration of the mussel Mytilus edulis by the trematode parasite Prosorhynchus squamatus: specificity and partial characterization of endogenous and parasiteinduced anti-mitotic activities. Comparative Biochemistry and Physiology, 1993, 104A (2), 229–233. [CrossRef] [Google Scholar]
- Dickerman E.E. Paurorhynchus hidionts a new genus and species of trematoda (Bucephalidae, Paurorhynchinae n. subfam.) from the mooneye fishes Hiodon tergisus. Journal of Parasitology, 1954, 40, 311–315. [CrossRef] [Google Scholar]
- Dfiwel D. Unusually large eggs of a Fasciola hepatica strain. Z. Parasitenkd, 1982, 67, 121–124. [CrossRef] [PubMed] [Google Scholar]
- Etchegoin A.J., Timi T.J., Cremonte F. & Lanfranchi L.N.A. Redescription of Prosorhynchus australis Szidat, 1961 (Digenea, Bucephalidae) parasitizing Conger orbignianus Valencienne, 1842 (Pisces, Congridae) from Argentina. Acta Parasitologica, 2005, 50 (2), 102–104. [Google Scholar]
- Francisco C.J., Hermida M.A. & Santos M.J. Parasites and Symbionts from Mytilus galloprovincialis (Lamark, 1819) (Bivalves: Mytilidae) of the Aveiro Estuary Portugal. Journal of Parasitology, 2010, 96 (1), 200–205. DOI: 10.1645/GE-2064.1. [CrossRef] [Google Scholar]
- Galaktionov K.V. & Dobrovolskij A.A. The biology and evolution of trematodes: an essay on the biology, morphology, life cycles, transmissions, and evolution of digenetic trematodes. Kluwer Academic Publishers, Netherlands, Boston, London, 2003. [Google Scholar]
- Jones O.D. The anatomy of three digenetic trematodes, Skrjabiniella aculeatus (Odhner), Lecithochirium rufoviride (Rud.) and Sterrhurus fusiformis (Lühe) from Conger conger (Linn). Parasitology, 1943, 35 (1–3), 40–57. [CrossRef] [Google Scholar]
- Kearn G.C. Parasitism and the platyhelminthes. Chapter 13 – The biology of Fasciola hepatica. Springer , 1997, 297. [Google Scholar]
- Laffargue P., Baudouin G., Sasal P., Arnaud C., Anras Marielaure B. & Lagardere F. Parasitic infection of sole Solea solea by Prosorhynchus spp. metacercariae (Digenea: Bucephalidae) in Atlantic nurseries under mussel cultivation influence Disease of Aquatic Organisms, 2004, 58 (2–3), 179–184. [CrossRef] [Google Scholar]
- Matthews R.A. The life-cycle of Prosorhynchus crucibulum (Rudolphi, 1819) Odner, 1905, and a comparison of its cercaria with that of Prosorhynchus squamatus Odhner, 1905. Parasitology, 1973, 66, 133–164.. [CrossRef] [Google Scholar]
- McGladdery S.E., Stephenson M.F. & Mcarthur F. Prosorhynchus squamatus (Digenea: Platyhelminthes) infection of bleu mussels, Mytilus edulis, in Atlantic Canada Journal of Shellfish Research, 1999, 18, 297 (Abstract) [Google Scholar]
- Santos M.J. & Gibson D.I. Morphological features of Prosorhynchus crucibulum and P. aculeatus (Digenea: Bucephalidae), intestinal parasites of Conger conger (Pisces: Congridae), elicited by scanning eletron microscopy. Folia Parasitologica, 2002, 49, 96–102. [PubMed] [Google Scholar]
- Suloeva T.A., The patterns of organization of different phases of the bucephalids (Trematoda: Bucephalidae) life cycle St. Petersburg State University, St. Petersburg, Russia, 1999. [Google Scholar]
- Woodhaed A.E. Life history studies on the trematode family Bucephalidae II. Transactions of the American Microscopical Society, 1930, 49, 1–17. [CrossRef] [Google Scholar]
- Winter H.A. Algunos trematodos digenea de peces marinos de aguas del Oceano Pacifico del sur de California USA y del litoral Mexicano. Anales del Instituto de Biologia Universidad Nacional Autonoma de México, 1960, 183–208. [Google Scholar]
Miracidium of Fasciola hepatica (Linnaeus, 1758) redrawn and adapted from Kearn (1997).
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