Table II.
Survival, brain cysts and PCR of BALB/c mice primary infected with the D8 or ME49 strains, immunosuppressed or non-immunosuppressed with cyclophosphamide (Cy), and challenged 45 days after primary infection with the CH3 or EGS strains of Toxoplasma gondii.
Reinfection |
PCR-RFLPe n/N (%) |
|||||
---|---|---|---|---|---|---|
Groupa | Strains/Immunosuppressionb | Survivalc n/N (%) | Cyst numberd | cS10-A6 locus | L363 locus | |
BALB/c mice primary infected with D8 strain of T. gondii | 1 | D8+CH3 | 10/10 (100) | 230,0 ± 127,4 | 0/10 (0) | ND |
2 | D8+CH3/Cy | 10/10 (100) | 365,0 ± 219,9 | 0/10 (0) | ND | |
3 | D8+EGS | 10/10 (100) | 135,0 ± 66,9f | 9/10 (90) | ND | |
4 | D8+EGS/Cy | 4/10 (40) | 662,5 ± 579,3f | 2/4 (50) | ND | |
5 | D8 | 10/10 (100) | 185,0 ± 91,4 | ND | ND | |
6 | D8/Cy | 10/10 (100) | 140,0 ± 69,9 | ND | ND | |
7 | CH3 | 0/10 (0) | NS | ND | ND | |
8 | EGS | 0/10 (0) | NS | ND | ND | |
9 | Naive/Cy | 10/10 (100) | 0 | ND | ND | |
BALB/c mice primary infected with ME49 strain of T. gondii | 10 | ME49+CH3 | 9/9 (100) | 551,1 ± 270,9 | ND | 4/9 (44,4) |
11 | ME49+CH3/Cy | 7/9 (77,8) | 350,0 ± 155,5 | ND | 5/7 (71,4) | |
12 | ME49+EGS | 8/9 (88,9) | 2543,7 ± 3646,9g | ND | 8/8 (100) | |
13 | ME49+EGS/Cy | 0/9 (0) | NS | ND | NS | |
14 | ME49 | 8/8 (100) | 312,5 ± 138,2g | ND | ND | |
15 | ME49/Cy | 7/8 (87,5) | 250,0 ± 141,4 | ND | ND | |
16 | CH3 | 0/9 (0) | NS | ND | ND | |
17 | EGS | 0/9 (0) | NS | ND | ND | |
18 | Naive/Cy | 9/9 (100) | 0 | ND | ND |
Experimental groups 1–18 are described on details in “Material and Methods”. The experiments were repeated twice and provided similar results.
It indicates: strain of primary infection + strain of challenge (when performed) / Cy treatment (when performed). Naive: mice without infection.
Number of mice presenting the two strains (n) (positive PCR at cS10-A6 or L363 locus) out of the total number of survivors after challenge (N).
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