Survival, brain cysts and PCR of BALB/c mice primary infected with the D8 or ME49 strains, immunosuppressed or non-immunosuppressed with cyclophosphamide (Cy), and challenged 45 days after primary infection with the CH3 or EGS strains of Toxoplasma gondii.
||PCR-RFLPe n/N (%)
|Groupa||Strains/Immunosuppressionb||Survivalc n/N (%)||Cyst numberd||cS10-A6 locus||L363 locus|
|BALB/c mice primary infected with D8 strain of T. gondii||1||D8+CH3||10/10 (100)||230,0 ± 127,4||0/10 (0)||ND|
|2||D8+CH3/Cy||10/10 (100)||365,0 ± 219,9||0/10 (0)||ND|
|3||D8+EGS||10/10 (100)||135,0 ± 66,9f||9/10 (90)||ND|
|4||D8+EGS/Cy||4/10 (40)||662,5 ± 579,3f||2/4 (50)||ND|
|5||D8||10/10 (100)||185,0 ± 91,4||ND||ND|
|6||D8/Cy||10/10 (100)||140,0 ± 69,9||ND||ND|
|BALB/c mice primary infected with ME49 strain of T. gondii||10||ME49+CH3||9/9 (100)||551,1 ± 270,9||ND||4/9 (44,4)|
|11||ME49+CH3/Cy||7/9 (77,8)||350,0 ± 155,5||ND||5/7 (71,4)|
|12||ME49+EGS||8/9 (88,9)||2543,7 ± 3646,9g||ND||8/8 (100)|
|14||ME49||8/8 (100)||312,5 ± 138,2g||ND||ND|
|15||ME49/Cy||7/8 (87,5)||250,0 ± 141,4||ND||ND|
Experimental groups 1–18 are described on details in “Material and Methods”. The experiments were repeated twice and provided similar results.
It indicates: strain of primary infection + strain of challenge (when performed) / Cy treatment (when performed). Naive: mice without infection.
Number of mice presenting the two strains (n) (positive PCR at cS10-A6 or L363 locus) out of the total number of survivors after challenge (N).
Significant difference between mice primary infected with the D8 strain and challenged with the EGS strain and mice primary infected with the D8 strain, Cy-immunosuppressed and challenged with the EGS strain, p < 0.05.
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