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Table II.

Survival, brain cysts and PCR of BALB/c mice primary infected with the D8 or ME49 strains, immunosuppressed or non-immunosuppressed with cyclophosphamide (Cy), and challenged 45 days after primary infection with the CH3 or EGS strains of Toxoplasma gondii.

Reinfection
PCR-RFLPe n/N (%)
Groupa Strains/Immunosuppressionb Survivalc n/N (%) Cyst numberd cS10-A6 locus L363 locus
BALB/c mice primary infected with D8 strain of T. gondii 1 D8+CH3 10/10 (100) 230,0 ± 127,4 0/10 (0) ND
2 D8+CH3/Cy 10/10 (100) 365,0 ± 219,9 0/10 (0) ND
3 D8+EGS 10/10 (100) 135,0 ± 66,9f 9/10 (90) ND
4 D8+EGS/Cy 4/10 (40) 662,5 ± 579,3f 2/4 (50) ND
5 D8 10/10 (100) 185,0 ± 91,4 ND ND
6 D8/Cy 10/10 (100) 140,0 ± 69,9 ND ND
7 CH3 0/10 (0) NS ND ND
8 EGS 0/10 (0) NS ND ND
9 Naive/Cy 10/10 (100) 0 ND ND

BALB/c mice primary infected with ME49 strain of T. gondii 10 ME49+CH3 9/9 (100) 551,1 ± 270,9 ND 4/9 (44,4)
11 ME49+CH3/Cy 7/9 (77,8) 350,0 ± 155,5 ND 5/7 (71,4)
12 ME49+EGS 8/9 (88,9) 2543,7 ± 3646,9g ND 8/8 (100)
13 ME49+EGS/Cy 0/9 (0) NS ND NS
14 ME49 8/8 (100) 312,5 ± 138,2g ND ND
15 ME49/Cy 7/8 (87,5) 250,0 ± 141,4 ND ND
16 CH3 0/9 (0) NS ND ND
17 EGS 0/9 (0) NS ND ND
18 Naive/Cy 9/9 (100) 0 ND ND
a

Experimental groups 1–18 are described on details in “Material and Methods”. The experiments were repeated twice and provided similar results.

b

It indicates: strain of primary infection + strain of challenge (when performed) / Cy treatment (when performed). Naive: mice without infection.

c

Number of survivors (n) out of the total number of mice challenged (N).

d

Mean number of brain cysts (and standard deviation) evaluated 30 days post challenge.

e

Number of mice presenting the two strains (n) (positive PCR at cS10-A6 or L363 locus) out of the total number of survivors after challenge (N).

f

Significant difference between mice primary infected with the D8 strain and challenged with the EGS strain and mice primary infected with the D8 strain, Cy-immunosuppressed and challenged with the EGS strain, p < 0.05.

g

Significant difference between mice primary infected with the ME49 strain and challenged with the EGS strain and mice primary infected with the ME49 strain, p < 0.05. NS: No survival; ND: Not done.

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