Relative expression levels (RExL.) of IFN-γ and IL-4 genes determined in mice 50 days post-immunization with pDRIVEEgAgB8/ 2 and empty plasmid (pDRIVE), using different routes of immunization (i.m., i.n. and g.g.).

mRNA was prepared from spleen cells and reverse transcribed into cDNA. The expression level of IFN-γ and IL-4 genes were amplified using specific primers and detected using SYBER-Green I using the real time PCR machine. Each block represents the average expression level of three mice from each group. Comparative C^T method (∆∆C^T) was used to calculate the expression level of each cytokine gene relative to β-actin gene as internal control as mentioned in material and methods section.