PCR detection of Anaplasma phagocytophilum in goat flocks in an area endemic for tick-borne fever in Switzerland

C. Silaghi; M.C. Scheuerle; L.M. Friche Passos; C. Thiel; K. Pfister

doi:10.1051/parasite/2011181057

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Volume 18 / No 1 (February 2011)

Parasite, 18 1 (2011) 57-62

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Original contribution

Table 2.

Comparison of the 526 bp sequences of the 16S rRNA gene of Anaplasma phagocytophilum from this study with selected sequences from the GenBank database.

	Nucleotide position^a
Accession no.	64	76	84	170	175	376	46133	463	464	465	467	468	Host	Country
U02521	A	A	G	C	C	G	C	T	G	C	A	G	Human	USA
FJ538288^b	•	G	A	•	•	•	•	•	•	•	•	•	Goat	CH^f
FJ538289^c	•	G	•	•	•	•	•	•	•	•	•	•	Goat	CH
FJ538290^d	•	•	•	T	•	A	•	•	•	•	•	•	Goat	CH
FJ538291	•	•	A	•	•	•	•	•	•	•	•	•	Cattle^e	CH, GER^g
M73220	•	•	A	•	•	•	•	•	•	•	•	•	Sheep	N^h
AF336220	G	•	A	•	•	•	G	G	A	T	C	C	Sheep	N
AF084907	•	•	•	•	•	•	•	•	•	•	•	•	I. ricinus	CH
AF384213	•	G	A	•	•	•	•	•	•	•	•	•	Roe deer	CH
AF384214	•	G	A	•	A	•	•	•	•	•	•	•	Roe deer	CH

^a

Nucleotide position in relation to the published prototype sequence of the HGA agent (U02521). Comparison was made for bp 59-584 of the prototype with the obtained 526 bp sequence from this study. Dots show identities with the prototype sequence;

^b

nuclotide sequence from goats B10 (June), M8 (October);

^c

nucleotide sequence from B11 (June);

^d

nucleotide sequence from A8 (July);

^e

identical sequence obtained from 13 cattle;

^f

CH, Switzerland;

^g

GER, Germany;

^h

N, Norway.

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