| Issue |
Parasite
Volume 28, 2021
|
|
|---|---|---|
| Article Number | 6 | |
| Number of page(s) | 7 | |
| DOI | https://doi.org/10.1051/parasite/2021002 | |
| Published online | 19 January 2021 | |
Research Article
The complete mitochondrial genome of Dermacentor (Indocentor) auratus (Acari, Ixodidae)
Le génome mitochondrial complet de Dermacentor (Indocentor) auratus (Acari, Ixodidae)
National Public Health Laboratory, National Centre for Infectious Diseases, Block G, Level 13, 16 Jalan Tan Tock Seng, Singapore 308442, Singapore
* Corresponding author: jmg_chavatte@ncid.sg
Received:
4
October
2020
Accepted:
6
January
2021
Dermacentor (Indocentor) auratus Supino, 1897 is a prominent ixodid vector of numerous pathogens of public health and veterinary importance. Using long-range PCR of two overlapping regions sequenced on an Illumina MiSeq machine, the complete mitochondrial genome of D. auratus is reported here. The resulting contigs were able to be assembled into a complete and circularised genome which had the general organisation of the mitochondrial genomes of the Metastriates. It had a total length of 14,766 bp and contained 37 genes, including 13 protein-coding genes, 22 transfer RNA genes, and 2 ribosomal RNA genes, as well as 2 non-coding control regions and 3 tick-boxes. The phylogenetic analysis on the whole mitogenome confirmed the position of D. auratus within the Dermacentor clade.
Résumé
Dermacentor (Indocentor) auratus Supino, 1897 est un ixode, vecteur notable de nombreux pathogènes importants en santé publique et en médecine vétérinaire. Utilisant la PCR en fragment long sur deux régions chevauchantes combinée au séquençage sur une plate-forme Illumina MiSeq, le génome mitochondrial complet de D. auratus est présenté ici. Les contigs obtenus ont été assemblés en un génome circulaire complet conforme à l’organisation générale des génomes mitochondriaux des Métastriates. D’une longueur totale de 14766 pb, il contient 37 gènes, incluant 13 gènes codants des protéines, 22 gènes d’ARN de transfert et 2 gènes d’ARN ribosomaux, ainsi que 2 régions contrôles non-codantes et 3 motifs « tick-box ». L’analyse phylogénétique sur le mitogénome complet confirme la position de D. auratus au sein du clade Dermacentor.
Key words: Dermacentor (Indocentor) auratus / Mitochondrial genome / Long-range PCR / Illumina sequencing
© J.-M. Chavatte & S. Octavia, published by EDP Sciences, 2021
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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